Expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein in eukaryotic cells

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cloning and expression of the g1 epitope of bovine ephemeral fever virus g glycoprotein in escherichia coli

bovine ephemeral fever (bef) is an acute epidemic disease in cattle and water buffalo, spanning tropical and subtropical zones of asia, australia, and africa. in recent years, bef has been distributed in many provinces of iran and caused economic losses. in this study the sequence encoding the g1 epitope of bovine ephemeral fever virus (befv) g glycoprotein was amplified by pcr, ligated into th...

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Expression of G1- epitope of bovine ephemeral fever virus in E. coli : A novel candidate to develop ELISA kit

Bovine ephemeral fever is an acute and arthropod-borne viral disease of cattle and water buffalo which occurs seasonally in most of the world tropical and subtropical regions. The epizootic feature of the disease has been reported in Iran with serious economic consequences. The surface glycoprotein G of bovine ephemeral fever virus (BEFV) is composed of 4 antigenic sites (G1-G4) and plays the m...

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Cloning and Expression of the G1 Epitope of Bovine Ephemeral Fever Virus G Glycoprotein in Escherichia Coli

Bovine ephemeral fever (BEF) is an acute epidemic disease in cattle and water buffalo, spanning tropical and subtropical zones of Asia, Australia, and Africa. In recent years, BEF has been distributed in many provinces of Iran and caused economic losses. In this study the sequence encoding the G1 epitope of bovine ephemeral fever virus (BEFV) G glycoprotein was amplified by PCR, ligated into th...

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Production of Monoclonal Antibody against Prokaryotically Expressed G1 Protein of Bovine Ephemeral Fever Virus

Epitope-G1 of bovine ephemeral fever virus (BEFV) G glycoprotein has been genetically and antigenically conserved among various isolates of BEFV and only reacts with anti-BEFV neutralising antibodies. Therefore, it is a candidate antigen for development of the enzyme linked immunosorbent assay (ELISA) for serological identification bovine ephemeral fever (BEF)-infected animals. The aim of this ...

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cloning and sequencing of g glycoprotein gene of bovine ephemeral fever virus in escherichia coli

introduction bovine ephemeral fever (bef) is an arthropod-borne viral disease of cattle and water buffalo, spanning tropical and subtropical zones in asia, australia, and africa continents. the clinical signs of bef disease in cattle are acute febrile reaction, stiffness, lameness, depression, cessation of rumination, and constipation. this disease was caused by ephemerovirus of the rhabdovirid...

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ژورنال

عنوان ژورنال: Bulgarian Journal of Veterinary Medicine

سال: 2018

ISSN: 1311-1477,1313-3543

DOI: 10.15547/bjvm.1061